12/10/2023 0 Comments Making mushroom spore prints![]() ![]() Before we do this we need to make sure our scalpel is sterile. Once the sterile distilled water is down to room temperature we will be scraping the spores off the foil into the water using our scalpel. Remove the sterile syringes you are using to fill with the spore solution and place them in the SAB along with the sterile needle and sterile syringe cap. the back of the foil the spore print is on.Clean down the items you will be working with using the alcohol and place them in your SAB. Once the lysol/ dettol has settled, get your surgical mask and gloves on and give your gloves a clean with your 70% alcohol solution. Clean your still air box with 70% isopropyl alcohol and then spray with lysol/ dettol for good measure. If you want to learn how to make this piece of equipment and why you would use it you can learn that here. We will be making the spore syringes in a still air box as these are very accessible. In the mean time we can start preparing our area and equipment. Putting your spores in hot water will kill them off and make them inactive – not very productive! ![]() Once your distilled water has finished sterilising we need to leave it to cool down to room temperature before we can begin making our spore syringes. Put your jar into your pressure cooker, wait for the pressure cooker to reach 15psi before leaving for 30 minutes. Put the distilled water into your flask/ jar and cover the top with tin foil. If you use a flask, and your needle is not long enough, it is very awkward trying to draw your spore solution out. By the same token you do not have to worry about how tall the mason jar is as the mouth is wide enough to get your syringe and hand into a mason jar to draw the spore solution out. Why use a mason jar instead of a flask?Īlthough a laboratory flask looks fancy and professional we prefer using a mason jar for the following reasons. It is easier to scrape the spores into a mason jar because the mouth is wider. The personal preference comes in as some people like spore syringes that are loaded with spores so that they can physically see the spores without using a microscope. As a general guide we would advise that one small (approximately 1cm diameter) and fairly faint print will only be good for one 10ml syringe while a larger dense print could create 30 x 10ml spore syringes. Another factor is the density and size of the spore print. This really comes down to personal preference as in reality each spore print is loaded with millions of spores. How many spore syringes can I make with a spore print? Multiply the volume of the syringes by the number of syringes and add on 3ml for good measure to take into account for difficulty getting every last drop of your spore solution out of the flask/ mason jar.īefore we go through the process of making a spore syringe, lets look at a couple of questions worth considering before we get started. Depending on how many syringes you are making from your spore prints will determine how much water you will need. Syringes for mycology generally range in size from 3ml up to 20 ml. Decide before hand how many spore syringes you are likely to make so that you can sterilise the correct amount of water. It makes sense to use the best quality water to begin with. I like to use a nice distilled water such as this one. To begin with we need to make sure the water we are using for our spore syringe is completely sterile. Leave for a couple of days before using.Suck the fluid back into the syringe and cap it.Draw the spore fluid up into the syringe and deposit back again to make sure the spores are evenly dispersed.Scrape the spores off your spore print using the scalpel into your sterile water. ![]()
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